Precise references for research peptides.
Plain reconstitution math, handling notes, and the dosing ranges reported in published research. No hype, no filler. Made for people who like to measure twice before they mix.
● For Research Use Only, not for human consumptionNew here? Start with the beginner's guide.
Featured compound references
Handling, storage, and the research behind each compound. One page each. Use the dropdown up top to jump straight to any of them.
Built from the research, not the noise
Most peptide protocols floating around online come from blogs, forums, or clinic marketing, with no citations and no accountability. Every reference here is built from published research and labeling, so you are working from real data, not someone's best guess.
- Every figure sourced from published literature and labeling
- No clinic marketing or unsourced forum claims
- Plain, consistent reconstitution instructions
- Dosage math you can check yourself on every page
- Reviewed and updated as new research lands
We cite primary sources directly, so you can verify everything yourself.
Essential guides
New to peptides? These cover the fundamentals every researcher needs.
Beginner's guide to peptides
What peptides are, how they work, the main categories, handling, and how to use this site.
How to reconstitute peptides
The reconstitution process step by step, bac water, concentration math, and worked examples.
Syringe & measurement guide
How to read U-100, U-50, and U-20 syringes. IU vs mg vs mcg vs syringe units explained.
Reconstitution calculator
Enter your vial, your diluent, and a target amount. Read back the draw volume and where it lands on a U-100 insulin syringe.
On a U-100 syringe, 100 units is 1 mL. This is reconstitution math only, not a dosing recommendation.
Ready to explore more?
Browse the compound library, run the numbers in the calculator, start with the beginner's guide, or read the latest on the blog.
Peptides from the ground up
If peptides keep turning up on your feed and you are trying to work out what they actually are, how they work, and what all the shorthand means, this is the place to begin. It walks through the basics, from the biology to the practical handling, without assuming you already know any of it.
What are peptides?
Peptides are short chains of amino acids, the same building blocks that make up proteins. The only real difference is length. Chains of roughly 2 to 50 amino acids are called peptides, and longer chains are proteins. Your body makes thousands of them, and they act as signals, hormones, and regulators for all kinds of processes.
A few you already know are peptides: insulin, which manages blood sugar, oxytocin, tied to bonding, and growth hormone, which is technically a protein but gets grouped in with the rest. When the research world says peptides, it usually means lab made copies of natural peptides, or new analogs designed to act like them, sold as freeze dried powder for research.
How peptides work
A peptide works by fitting a specific receptor on or inside a cell and setting off a response. Picture a key in a lock. The peptide is the key, the receptor is the lock, and the effect is what happens when the door opens.
Different peptides fit different locks, which is why their effects vary so much. A growth hormone releasing peptide binds ghrelin receptors in the pituitary to trigger a GH pulse. A GLP-1 agonist like semaglutide binds GLP-1 receptors that shape insulin and appetite. BPC-157 touches several pathways tied to repair and inflammation.
The idea to hold onto is specificity. Each peptide has a target, a dose range where it is active, and a half life that sets how long it stays active. That is why dosing matters. The same peptide at the wrong dose, the wrong timing, or the wrong route can do nothing, do something different, or cause side effects.
The main categories
The peptides studied today span a wide range of biological targets. These are the groups you will run into most.
Recovery and tissue repair
Studied for wound healing, tendon and ligament repair, gut healing, and lowering inflammation. The two most discussed are BPC-157, a 15 amino acid peptide derived from gastric protective proteins, and TB-500, a synthetic fragment of thymosin beta 4. They are often used together.
Growth hormone and secretagogues
This group either is growth hormone or prompts your body to release more of its own. The stimulators split into GHRH analogs, which amplify natural GH pulses (CJC-1295, sermorelin, tesamorelin), and ghrelin mimetics, which trigger a pulse through the ghrelin receptor (ipamorelin and others). The two types are often combined, like CJC-1295 with ipamorelin, because they work through complementary paths.
Metabolic and weight management
GLP-1 receptor agonists are the fastest growing category. They mimic the incretin hormone GLP-1 to shape appetite, blood sugar, and body weight. The headline names are semaglutide, tirzepatide, a dual GIP and GLP-1 agonist, and retatrutide, a triple agonist hitting GLP-1, GIP, and glucagon. These are among the most heavily studied peptides in clinical medicine today.
Skin, cosmetic, and anti-aging
Studied for skin quality, pigmentation, collagen, and aging. Examples include GHK-Cu, a copper peptide studied for skin remodeling and wound healing, and Melanotan, a melanocortin agonist studied for pigmentation.
Hormonal and sexual health
Peptides that touch reproductive and sexual pathways. HCG supports testosterone production in clinical use, and PT-141 is an approved melanocortin agonist for low sexual desire.
How peptides are supplied
Research peptides show up as a freeze dried powder, a small white cake at the bottom of a sealed glass vial with a rubber stopper and a metal cap. The label lists the name and the total amount, like BPC-157 5 mg. It is not ready to use as is. First it has to be reconstituted, or dissolved in a liquid, to make an injectable solution. The powder form exists because peptides last far longer dry than in liquid.
Reconstitution step by step
Reconstitution is the one practical skill that matters most. Here is the shape of it. What you need: the peptide vial, bacteriostatic water, an insulin syringe, and alcohol swabs.
- Wipe both rubber stoppers, the peptide and the bac water, with an alcohol swab.
- Draw your chosen amount of bac water into the syringe. More water means a more dilute mix and more liquid per dose.
- Push the water in slowly, aiming the stream at the glass wall rather than straight onto the powder.
- Swirl gently, never shake. Most powders dissolve in a minute or two, though some take up to ten.
- Put it in the fridge. It is now ready to use.
Every compound page lists the exact water amount for that vial, picked to make the dose math clean, and the calculator works it out for any amount you choose.
Why bac water, not plain water
The benzyl alcohol in bacteriostatic water holds off bacteria, so a mixed vial stays usable for many doses across roughly 25 to 30 days. Plain sterile water has no preservative, so you would have to use the whole vial within a day or two. For anything multi dose, bac water is the right pick.
Units and measurements
Mixing up units is the number one beginner mistake. Three systems are in play, and they measure different things.
Weight: mg and mcg
These measure how much peptide you have by weight. 1 mg is 1,000 mcg. Some peptides are dosed in milligrams, like semaglutide at 0.25 mg, and others in micrograms, like ipamorelin at 200 mcg. Swap them and you are off by a factor of 1,000.
Volume: mL and syringe units
These measure how much liquid you pull into the syringe. On a U-100 insulin syringe the marks run 0 to 100 units, where 100 units is 1 mL, so one unit is 0.01 mL. These are volume marks, not dose marks. They tell you nothing about how much peptide is in that volume until you know the concentration.
Potency: IU
International units measure biological strength for a few peptides, mainly HGH, at about 3 IU per mg, and HCG. IU are not the same as syringe units. When a protocol says inject 2 IU, it means potency, and you still work out the volume from your concentration.
Putting it together
A worked example. You have a 5 mg vial of BPC-157 and add 2 mL of bac water. The concentration is 5 mg divided by 2 mL, or 2.5 mg per mL. Your target is 250 mcg, which is 0.25 mg. So 0.25 divided by 2.5 is 0.10 mL, which is 10 units on a U-100 syringe. If that feels like a lot, the calculator does it for you.
Syringes
Almost every protocol uses insulin syringes, since they are built for small, precise volumes with thin needles.
- U-100, 1 mL: 100 units per mL, the common choice. Each unit is 0.01 mL.
- U-100, 0.5 mL: the same scale but it holds half as much, so the marks are more spread out and easier to read for small doses.
- U-50 and U-20: less common, with finer marks for very small volumes.
Our compound pages tell you the units to draw, and the calculator shows the exact mark for your vial.
Storage
Before reconstitution, the dry powder keeps best in the fridge at 2 to 8 °C for the long haul. Room temperature is fine for short stretches, but heat and light break peptides down faster, and many can be frozen for extended storage. Keep it out of direct sun and humidity.
After reconstitution, the liquid should always be refrigerated at 2 to 8 °C, and most mixed peptides hold for about 25 to 30 days. Never freeze a reconstituted peptide, since ice crystals can snap the chain. Keep the vial upright, do not shake it, and mark the date you mixed it.
Routes of administration
Most research protocols use one route.
Subcutaneous
Into the fatty layer just under the skin, usually the lower belly, the thigh, or the upper arm. It is the default for most peptides because absorption is reliable and it only needs a short, thin needle.
Intramuscular
Straight into muscle, called for by a few compounds like HCG. It needs a longer needle and a different technique, and it tends to absorb faster.
Oral and nasal
Most peptides survive the gut poorly, so oral forms are rare. A few exceptions exist, like oral semaglutide with a special absorption helper. Nasal delivery is studied for some peptides but is less common.
Safety
Every compound carries risk. A few principles hold across the board.
Side effects depend on the peptide. There is no single profile for peptides as a group. Common threads include injection site reactions, headaches, nausea, and fatigue. GH related peptides can bring water retention, joint pain, and shifts in blood sugar. GLP-1 agonists often cause gut effects. Each compound page has its own safety notes.
Dose matters, and more is not better. Peptides have a curve where the effect levels off while side effects keep climbing. Sticking to evidence based ranges, rather than forum megadose talk, is the biggest risk cut you can make.
Source quality is not guaranteed. Research use only material sits outside pharmaceutical oversight. A vendor certificate of analysis is not the same as regulated quality control, so purity, sterility, and accurate labeling are not assured. That is a real risk worth understanding.
Legal status varies. Some peptides are prescription drugs, some carry specific federal limits, many sit in a gray area as research chemicals, and most performance related ones are banned by WADA for tested athletes. See the FAQ for more.
Essential supplies checklist
- Peptide vials, dry, stored in the fridge
- Bacteriostatic water, usually a 10 mL or 30 mL vial
- Insulin syringes, U-100, 1 mL or 0.5 mL, 29 to 31 gauge
- Alcohol swabs for stoppers and skin
- A sharps container for used needles
- Fridge space for both dry and mixed vials
- Optional, small labels or tape to date your vials
How to use DosingReference
Compound library. A reference page for every compound we cover, with an overview, a sample dosing technique, reconstitution sized to the vial, storage, what the literature reports, and citations. It covers both single peptides and blends.
Calculator. Enter your vial size, your bac water amount, and your target dose, and it returns the exact syringe volume and how many doses the vial holds. It works for any compound.
FAQ. Quick answers on reconstitution, storage, legality, and using the site.
A suggested path
- Finish this guide to get the fundamentals down.
- Pick one compound that fits your interest and read its full page. BPC-157 is a common first stop, since it is widely studied and the handling is simple.
- Use the calculator to practice the math with that vial size and dose until the link between vial content, water, concentration, and units clicks.
- Check the FAQ for specific questions on storage, handling, and safety.
Frequently asked questions
General
What is DosingReference?
DosingReference is an independent educational reference for research peptides. We publish reconstitution guides, sample dosing techniques, and research backed background, drawn from published clinical literature, FDA labeling, and peer reviewed studies rather than forums or marketing copy.
Is this medical advice?
No. Everything here is for research and educational use only. Nothing on the site is meant to diagnose, treat, cure, or prevent any condition. Talk to a qualified healthcare professional before making any decision about peptides or other compounds.
Do you sell peptides?
No. DosingReference does not sell, ship, or supply peptides or any research material. We are an information site. Where supplier links appear, they are affiliate links to third party research use only vendors, Quartz Peptides among them, and we earn a commission on qualifying orders. That funding does not shape what we publish.
What are single peptides and blends?
A single peptide is one compound in a vial, like BPC-157 or retatrutide. A blend is two or more peptides pre mixed in one vial, like CJC-1295 with ipamorelin, which changes the dose math because a single solution holds more than one active ingredient. Our library covers both.
How do I request a compound you do not cover?
We add compounds regularly. If there is one you want to see, reach out with the name and vial size and we will look at adding it.
Reconstitution and dosing
What is reconstitution?
It is the step of adding a liquid, usually bacteriostatic water, to a freeze dried peptide powder to make an injectable solution. How much liquid you add sets the concentration, which sets how much you draw per dose.
What is bacteriostatic water?
Sterile water with 0.9% benzyl alcohol as a preservative. The benzyl alcohol holds off bacteria, so a mixed vial stays usable for many doses over days or weeks when stored right. It is the standard diluent for freeze dried peptides.
How much bacteriostatic water should I use?
It depends on the compound and vial size. Each compound page lists a suggested amount, usually picked to land on a round concentration that keeps the dose math simple, often 1, 2, or 3 mL. The calculator will work out the exact draw for any amount you choose.
What is the difference between IU and mg?
mg is weight, how much peptide powder is in the vial. IU, international units, measure biological strength for certain peptides like HGH and HCG, where weight and activity have been standardized. For HGH the rough conversion is about 3 IU per mg. Most peptides are not dosed in IU; they use mg or mcg.
What is the difference between mcg and mg?
1 mg is 1,000 mcg. Many peptides are dosed in micrograms because the active amounts are tiny. A 250 mcg dose is the same as 0.25 mg. Always check which one a protocol means, since mixing them up is a 1,000 fold error.
What syringe should I use?
Most protocols use U-100 insulin syringes, usually 0.5 mL or 1 mL. On a U-100 syringe 100 units is 1 mL, so each mark is 0.01 mL. Some small doses call for U-50 or U-20 syringes for finer measurement. Each compound page tells you which type and how many units to draw.
How do I use the calculator?
Enter the total peptide in your vial, the bac water you added, and your target dose. It returns the exact volume to draw and how many doses the vial holds. It works for any compound and vial size.
Storage and handling
How do I store peptides before reconstitution?
Keep dry, freeze dried peptides cool, dry, and out of light. The fridge at 2 to 8 °C is ideal for the long term, and many can be frozen for even longer. Check the specific compound's notes.
How do I store reconstituted peptides?
Once mixed with bac water, refrigerate at 2 to 8 °C and usually use within 25 to 30 days depending on the compound. Never freeze a mixed peptide, since ice crystals can degrade it. Keep the vial upright and do not shake it.
How long do reconstituted peptides last?
Most stay stable for about 25 to 30 days refrigerated with bac water, and some hold longer. If you used plain sterile water with no preservative, use it within a day or two.
Using our references
How do I read a compound page?
Each page follows the same shape: an overview of what the compound is and what it is studied for, a sample dosing technique, reconstitution sized to the vial, storage, what the literature reports, and references. The calculator lets you match any of it to your own vial.
Are your references peer reviewed?
The pages themselves are not peer reviewed like a journal article, but the dosing, mechanisms, and safety notes are drawn from peer reviewed literature, FDA labeling, clinical trial data, and standard pharmacology references, with citations on the page.
Safety and legal
Are peptides legal?
It depends on the country, the state, and the specific peptide. In the US many are sold as research chemicals not intended for human use. A few, like semaglutide, HGH, and HCG, are FDA approved prescription drugs in clinical use. Some carry specific limits; for example, federal law restricts HGH distribution for non medical human use. Check your local rules.
Are peptides banned in sport?
Many are prohibited by the World Anti-Doping Agency, including growth hormone, GH secretagogues like CJC-1295 and ipamorelin, GH fragments, and others. If you compete in a tested sport, check the current WADA Prohibited List first.
What are common side effects?
They vary a lot by compound. Common ones across many peptides are injection site reactions, headaches, nausea, and fatigue. GH related peptides can add water retention, joint pain, and blood sugar shifts. Each compound page has a compound specific safety section.
Can I combine peptides?
Some are commonly used together, and blends cover the pre mixed ones, but combining anything adds complexity and possible interactions. Where we note combinations, we say which have research behind them and which are more experimental. Talk to a healthcare professional before combining compounds.
Storing peptides the right way
Good storage is the difference between a peptide that performs and one that has quietly lost half its strength before the first dose. These are fragile molecules. Heat, light, moisture, and contamination all break them down. Here is how to store them correctly at every stage, from the day they arrive to the last draw in the vial.
Why storage matters
Peptides are amino acid chains held together by peptide bonds. Those bonds are stable in the right conditions but break down through a few routes: hydrolysis, where water splits the bond, oxidation, where oxygen damages certain residues, deamidation, where heat and moisture alter specific residues, and aggregation, where molecules clump and lose activity. None of these show an obvious visual change until the damage is severe, so a peptide can shed real potency while still looking perfectly fine.
The practical upshot is simple. A 5 mg vial stored badly might hold only 3 mg of active peptide by the time you mix it, while your dose math still assumes 5 mg. Every draw would be under dosed without you knowing. Correct storage prevents that silent loss.
Dry powder, before reconstitution
Freeze dried peptides sit in their most stable form. Pulling the water out slows every breakdown path, so stored well they last months to years.
Temperature
The fridge at 2 to 8 °C is the single most important condition, and a normal household fridge is perfect. There, most dry peptides stay stable for 12 to 24 months or more. Room temperature, 15 to 25 °C, is fine for short stretches like shipping and handling, but every day out spends some of the stability margin. For the long haul, the freezer at about -20 °C is safe for dry powder and stretches shelf life to a few years, since there is no water present to form damaging ice. Let a frozen vial reach room temperature before mixing, or condensation forms on the powder. Avoid anything above 25 °C. Heat is the main enemy. Car trunks, windowsills, and hot porches in summer all speed degradation, and even a few hours in real heat can cut potency for some peptides.
Light, moisture, and air
Keep it dark. UV and even visible light can oxidize certain residues, and most vials are clear glass with no built in protection, so leave them in their box or a dark part of the fridge. Keep it dry. Freeze drying removes water for a reason, and if a dry vial takes on humidity it can start to break down before you ever mix it, so keep the crimp cap intact and consider a desiccant packet in the storage bag. Keep it sealed. Do not pull the cap or stopper until you are ready to mix, since the seal keeps oxygen out. Once you puncture the stopper, that protection is gone, which is why mixed peptides last far less time.
How long it lasts
Refrigerated, most dry peptides hold 12 to 24 months. Frozen, often 2 to 3 years or more. At room temperature, weeks to a few months. Smaller, sturdier peptides like BPC-157 tend to handle heat better than large, complex ones. Follow any vendor expiry, and use older vials first.
Reconstituted peptides, the liquid solution
Once you add bac water, everything changes. The peptide is back in water and every breakdown path that freeze drying held off is active again. Mixed peptides need stricter handling and a firm expiry window.
Temperature
Always refrigerate at 2 to 8 °C, with no exceptions, and put it back right after mixing. A half hour on the counter while you measure is fine, but never leave it out for hours. Never freeze a mixed peptide. This is one of the most important rules in handling. When the solution freezes, ice crystals physically tear the peptide apart and wreck its structure, and the damage does not reverse. A mixed vial that has frozen should be discarded even if it looks normal. Watch fridge placement too, since the very back of some fridges dips below freezing. A middle shelf or a door compartment is usually safest.
Light and position
Keep it dark, which the fridge handles on its own, and wrap or bag a vial if you move it. Store it upright with the stopper on top. Lying on its side, the liquid sits against the rubber for long stretches, which can leach traces from the stopper or weaken the seal.
How long it lasts
With bac water and proper refrigeration, most mixed peptides hold 25 to 30 days, some longer, some less, and the compound pages note exceptions. With plain sterile water and no preservative, use within a day or two. Past the window, even if it still looks clear, discard the rest and mix a fresh vial. The potency loss is invisible.
Bacteriostatic water
Bac water has its own rules people tend to skip. Sealed, it is fine at room temperature and does not need the fridge. After the first puncture, the benzyl alcohol keeps it usable for about 28 days, and some people refrigerate opened bac water as extra insurance. Swab the stopper before every use, the same as a peptide vial. And it should always look crystal clear. If it turns cloudy or you see particles, throw it out.
Traveling with peptides
Keeping the cold chain matters most for mixed peptides. For short trips under about four hours, a small insulated bag or lunch cooler with an ice pack works, but wrap the pack in a paper towel so the vials do not touch it and freeze. For longer transport, use a hard insulated container with gel packs and a barrier between the packs and the vials, and a small thermometer if you can manage one. For flying, keep peptides in your carry on, never checked, since cargo holds swing to extremes on the tarmac and at altitude. Pack them in an insulated pouch with a small gel pack. Dry powder is more forgiving in transit because it tolerates short room temperature spells, so most vendors ship it with basic insulated packaging. Refrigerate as soon as it arrives.
Signs of degradation
Before mixing, in the powder: fresh peptide is white to off white, so yellowing or browning points to oxidation or heat damage. A collapsed cake on its own is not always a problem, since shipping vibration can do it, but paired with color change or moisture it is a concern. Droplets or a wet look before you add any liquid mean the seal may have failed and moisture got in. A damaged crimp cap or a stopper that shifts when pressed means the vial is no longer sealed.
After mixing, in the solution: it should be clear, so lasting cloudiness after gentle swirling suggests clumping or contamination. Visible particles can be aggregated peptide, stopper fragments, or contamination, so discard. Any color at all points to degradation. A strong or foul smell suggests bacteria. And unusual redness, swelling, or pain at an injection site beyond the norm for that compound can flag a bad solution. When in doubt, throw it out. A replacement vial costs less than the risk of using a degraded one.
Quick reference
| Stage | Temperature | Duration | Key rules |
|---|---|---|---|
| Dry powder, fridge | 2 to 8 °C | 12 to 24 months | Sealed, dark, dry. The best default. |
| Dry powder, freezer | about -20 °C | 2 to 3 years or more | Safe for powder. Warm to room temp before mixing. |
| Dry powder, room temp | 15 to 25 °C | Weeks to a few months | Short term only. Keep off heat and sun. |
| Mixed, bac water | 2 to 8 °C | 25 to 30 days | Refrigerate at once. Never freeze. Store upright. |
| Mixed, sterile water | 2 to 8 °C | 24 to 48 hours | No preservative, so use fast or discard. |
| Bac water, opened | Room temp or fridge | 28 days after first puncture | Swab the stopper every time. |
Best practices
- Refrigerate by default. Both dry and mixed peptides belong in the fridge.
- Never freeze a mixed peptide. Ice crystals destroy it, and it does not reverse.
- Freezing dry powder is fine for the long term. Warm the vial to room temperature before mixing.
- Keep vials dark. Inside a closed fridge is ideal.
- Keep the seal until you are ready to mix.
- Label every mixed vial with the date and the bac water volume, and discard after 25 to 30 days regardless of looks.
- Store mixed vials upright.
- Swab every stopper with alcohol before each needle, peptide and bac water alike.
- Use bac water for multi dose vials. Sterile water is only for a vial you will use in one session.
- When in doubt, discard. Cloudy, colored, or smelly solutions should never be used.
Reading a syringe without the guesswork
Measurement mistakes are the most common error in peptide work, and almost all of them trace back to one thing: confusing different kinds of units. This runs through every syringe type you will meet, how to read them, how the unit systems relate, and the conversion math that ties it together.
Insulin syringes, the standard tool
Nearly every protocol uses insulin syringes. They are built for small, precise volumes with thin needles, usually 29 to 31 gauge, that suit subcutaneous injection. They are cheap, easy to find, and come with a fixed needle, which means less liquid wasted in the hub than syringes with detachable needles. They are labeled by two things: total capacity, how much they hold, and unit scale, what the tick marks mean. The scale is where confusion starts.
Types and scales
U-100, 1 mL
The most common one. It has 100 unit marks along the barrel, where 100 units is 1 mL, so each unit is 0.01 mL. The smallest graduation is usually 2 units. Best for 10 to 100 units of liquid. This is the default our calculator outputs.
U-100, 0.5 mL
Same scale, 100 units per mL, but it holds 50 units. The marks are spread further apart, so small volumes read more precisely, usually one mark per unit. Best for 5 to 50 units, and often the better pick for doses under 30 units.
U-100, 0.3 mL
The smallest common one, holding 30 units. The most spread out marks, often with half unit marks, so the most precise read for tiny doses. Best for 1 to 30 units, ideal for high concentration mixes or low microgram doses.
U-50 and U-20
Less common. On a U-50, 50 units is 0.5 mL, and each unit is still 0.01 mL, so the volume at any unit mark matches a U-100. The difference is capacity and mark spacing, and where a page mentions U-50 the volume math is the same as U-100. U-20 is mostly veterinary, occasionally referenced for very small doses, with 20 units at 0.2 mL and the widest spacing.
How to read one
- Identify the type. The barrel or box says U-100, U-50, or U-20 and the capacity. U-100 and 1 cc, which is the same as 1 mL, is the common one.
- Understand the lines. On a U-100 syringe the numbers usually sit every 10 units with shorter lines between. On the 1 mL those between lines are often 2 unit steps, and on the 0.5 and 0.3 mL they are usually single units, with the 0.3 mL often adding half unit marks.
- Read at the plunger tip. Read at the top flat edge of the rubber stopper, the side toward the needle, not the domed bottom, and hold it at eye level.
- Deal with air bubbles. Bubbles displace liquid and throw off the reading. Point the needle up, flick to float them, push the plunger to expel the air, and top up if needed.
The three unit systems
This is where beginners trip. Three different systems, measuring three different things.
Weight: mg and mcg
The mass of peptide. This is what the vial label shows and what the target dose is in. 1 mg is 1,000 mcg, and micrograms are sometimes written µg. Always confirm whether a protocol means mg or mcg, since mixing them is a 1,000 fold error.
Volume: mL and syringe units
How much liquid you draw. 1 mL is 100 units on a U-100 syringe, so 1 unit is 0.01 mL, and mL and cc are the same thing. Syringe units are purely volume marks. They are not international units and they say nothing about the dose until you know the concentration. Twenty units is 0.20 mL of liquid whatever is dissolved in it.
Potency: IU
Biological activity for a few standardized peptides, mainly HGH at about 3 IU per mg, and HCG, whose IU come from bioassay. Most peptides do not use IU. When a protocol says inject 2 IU of HGH, that is a potency amount, not a syringe mark, and you still work out the volume from your concentration.
Conversion math
Every calculation is the same three steps.
- Concentration: total peptide divided by bac water. Example, 5 mg plus 2 mL is 2.5 mg per mL.
- Dose volume: target dose divided by concentration. Example, 0.25 mg divided by 2.5 mg per mL is 0.10 mL.
- Syringe units: dose volume times 100 for U-100. Example, 0.10 mL times 100 is 10 units.
That is it, for every peptide and vial. The calculator runs all three instantly.
Quick tables
| mg | mcg |
|---|---|
| 0.1 mg | 100 mcg |
| 0.25 mg | 250 mcg |
| 0.5 mg | 500 mcg |
| 1 mg | 1,000 mcg |
| 2 mg | 2,000 mcg |
| 5 mg | 5,000 mcg |
| mL | U-100 units |
|---|---|
| 0.01 mL | 1 unit |
| 0.05 mL | 5 units |
| 0.10 mL | 10 units |
| 0.20 mL | 20 units |
| 0.25 mL | 25 units |
| 0.50 mL | 50 units |
| 1.00 mL | 100 units |
HGH, IU to mg to units, assuming a 10 IU vial mixed with 1 mL of bac water. A different water volume gives a different unit reading.
| IU | mg (approx.) | Units |
|---|---|---|
| 1 IU | 0.33 mg | 10 units |
| 2 IU | 0.67 mg | 20 units |
| 3 IU | 1.00 mg | 30 units |
| 4 IU | 1.33 mg | 40 units |
| 5 IU | 1.67 mg | 50 units |
| 10 IU | 3.33 mg | 100 units, full syringe |
Common mistakes
- Confusing syringe units with IU. The most dangerous one. Inject 2 IU of HGH does not mean the 2 mark. Work from concentration. A 10 IU vial mixed with 1 mL means 2 IU is 20 units, and with 2 mL it is 40 units. Same IU, different marks.
- Confusing mg and mcg. 250 mcg measured as 250 mg is a 1,000 fold overdose. Check the abbreviation, and convert when unsure: 250 mcg is 0.25 mg.
- Reading the wrong part of the plunger. Read the top flat edge, not the domed bottom, or you carry a 1 to 2 unit error every draw.
- Ignoring air bubbles. A 5 unit bubble in a 15 unit draw leaves only 10 units of liquid. Expel air first.
- Wrong syringe for the volume. Five units on a 1 mL syringe is nearly unreadable, so switch to a 0.3 or 0.5 mL. And 80 units will not fit a 0.5 mL. Match the syringe to the dose.
- Assuming every syringe shares one scale. Most are U-100, but U-40 veterinary syringes use a different relationship. Check the barrel before drawing.
Needle gauge and length
Gauge is thickness, and higher numbers are thinner. 29G draws thick solutions easily, 30G is the popular middle, and 31G is thinnest and most comfortable but slower to draw. Anything 29 to 31 works for subcutaneous use, and the comfort difference is small. For length, half an inch, about 12.7 mm, is the common subcutaneous choice, and 5/16 inch, about 8 mm, suits leaner sites. A common default is 30G by half an inch.
Which syringe to use
| Dose volume | Best syringe | Why |
|---|---|---|
| 1 to 30 units | U-100, 0.3 mL | Half unit marks, easiest to read tiny volumes. |
| 5 to 50 units | U-100, 0.5 mL | Single unit marks, a good balance of precision and capacity. |
| 10 to 100 units | U-100, 1 mL | Full capacity, the standard for reconstitution. |
For reconstitution, where you add 1 to 3 mL of water, use a 1 mL syringe. For daily dosing, use the smallest syringe that holds your dose for the best read.
Measurement checklist
- Confirm the dose and whether it is mg or mcg, or IU for HGH and HCG.
- Check your concentration, total peptide over bac water.
- Work out the dose volume, target over concentration.
- Convert to units, mL times 100 for U-100.
- Pick the right syringe size for that volume.
- Confirm it is U-100 on the barrel.
- Draw and check for bubbles.
- Read at the top flat edge at eye level.
- Confirm the reading matches your math before injecting.
Sourcing and standards
DosingReference publishes dosing references, reconstitution guides, and research backed background on peptides. This page lays out how that content is made, sourced, checked, and kept current, so you can judge it for yourself and decide how much weight to give it.
Our aim
The most accurate, clearly sourced, and genuinely useful peptide reference we can build. Most peptide information online comes from forums, social posts, clinic marketing, or vendor pages, usually with no citations, no read on how strong the evidence is, and no clear disclaimers. We exist to raise that bar. We are an educational publisher, not a clinic, a pharmacy, or a peptide seller. We do not diagnose, treat, prescribe, or sell. The content is here to help you see what the published evidence actually says, and where it runs out.
How we source
Every substantive claim, dosing ranges, mechanisms, side effects, pharmacokinetics, and safety, comes from one or more of these.
Primary sources, which we lean on first. Peer reviewed journal articles in indexed biomedical journals, including trials, clinical pharmacology studies, and reviews. FDA prescribing information and labeling for approved peptide products like semaglutide, tirzepatide, and tesamorelin. And trial registrations and results from registries like ClinicalTrials.gov, especially for compounds still in development.
Secondary sources, as support. Endocrine and specialty society guidelines, government and regulatory publications like FDA safety notices and the WADA list, and standard pharmacology references.
What we do not treat as sources. Forum and social posts, vendor marketing beyond plain product specs, anecdotes without published backing, unverified AI output, and other peptide sites unless they cite primary sources we can check ourselves.
How a page is built
- Research first. Before writing, we search the literature and the label databases, and for dosing we pull ranges from trials, prescribing information, and guidelines, favoring human data over animal data wherever it exists.
- Draft with citations in place. Sources go in at the point of each claim, not bolted on afterward. The evidence drives the content.
- Check accuracy. Dosing ranges are verified against the cited source, unit conversions are recomputed, and the reconstitution math is run a second time.
- Publish and keep watching. Pages are not final. We update them as new evidence lands, labels change, or errors surface.
Grading the evidence
Not all evidence is equal, and you deserve to see the difference. We separate strong evidence, backed by multiple trials, reviews, or approved labeling, which we state plainly. Moderate evidence, from limited trials, observational work, or expert consensus, which we frame as research suggests rather than settled fact. Preliminary or preclinical evidence, from animal or very early human work, which we flag as such and note the gap to proven human outcomes. And insufficient evidence, where a popular online claim lacks real published support, which we say directly instead of repeating it. Someone who knows a claim rests on a single mouse study can weigh it very differently from someone told it is proven.
Who writes it
Pages are published under our team byline, not attributed to invented experts, and we do not borrow credentials we cannot stand behind. We do not claim independent medical review. Where a page cites a researcher or a study, that means their published work is a source, not that they wrote, reviewed, sponsored, or endorsed our page. Each page shows its publication and update context.
Updates and corrections
We re review content over time, prioritizing compounds with active development where new data can shift dosing, high traffic pages, and anything a reader flags. If we find an error, whether a math slip, a bad citation, a misread study, or any other inaccuracy, we fix it promptly, and for anything that touches dosing or safety we note the change and its date at the top of the page. If you spot a problem, tell us with the page and a description and we will look into it.
Links and independence
External links serve two jobs, citations to the evidence behind a claim and cross references to regulatory or authoritative documents. They are for your verification and are not endorsements. Some pages also carry supplier links to third party research use only products, because readers often ask where to source material for research, and a reference is more useful than silence. Those are affiliate links, so we earn a commission on qualifying orders, including from Quartz Peptides. That funding is disclosed, and it does not buy editorial control. No vendor reviews or approves our content, no one pays for placement or a favorable write up, and we do not drop safety information to suit anyone. If the evidence shows a risk or a limit, it goes in.
What this is not
- Not a medical provider. Nothing here is medical advice, diagnosis, or treatment, and there is no patient relationship.
- Not a pharmacy. We do not sell, compound, or dispense anything.
- Not a research organization. We do not run trials, sponsor research, or collect patient data.
- Not a substitute for professional guidance. For any clinical or therapeutic question, consult a qualified healthcare professional.
Last reviewed in 2026.
About DosingReference
DosingReference is an independent educational reference for peptides. We built it because the information researchers actually need, clean reconstitution math, evidence based dosing ranges, and honest safety profiles, was scattered across forums, locked behind paywalls, or tangled up with unsourced marketing. We wanted to fix that.
Who is behind it
We are a small group of people who are genuinely excited about where peptide research is heading, and frustrated by how hard it is to find straight information about it. We publish under a shared team name rather than invented expert profiles or borrowed credentials. We do not claim independent medical review. When a page cites a study or a label, that citation is the source doing the work, and you can follow it yourself.
Why we exist
The peptide space has an information quality problem. Search any protocol and you get dozens of results that mostly share the same traits: no citations, no source links, and no way to tell whether a number came from a clinical trial or a forum post from years ago. Dosing tables get copied site to site with nobody checking the math. We started DosingReference to do the opposite. Every reference here is built from published literature, prescribing information, and standard pharmacology sources, with links so you can check it. Where the evidence is strong we say so, and where it is thin we say that too. Honest uncertainty beats false confidence.
What we publish
Compound references. A growing library covering single peptides and blends. Each page has an overview, a sample dosing technique, reconstitution sized to the vial, storage, what the literature reports, and citations.
Calculator. Enter any vial size, bac water amount, and target dose, and it returns the exact syringe volume and doses per vial.
Guides. Standalone walkthroughs for newcomers, covering the beginner basics, storage, and syringe measurement, plus an FAQ.
Blog. Longer pieces on peptide science, mechanisms, comparisons, and new research, each written from published sources.
What makes it different
Every claim is sourced. We do not publish a dose, a mechanism, or a safety note without a source, and if we cannot find credible published backing, the claim stays out.
We grade the evidence. Multiple trials read very differently from one animal study, and our language reflects that.
The math is shown. Every reference lays out the full chain, vial content, water, concentration, target dose, and the exact volume in mL and units, so anyone can check it.
It is maintained, not abandoned. Research moves, and we update rather than publish once and walk away.
No unsourced claims. We do not repeat forum lore or marketing copy as fact, which sometimes means disagreeing with what is popular. We are fine with that.
Who it is for
Anyone who wants accurate, sourced peptide information laid out clearly: independent researchers, students, professionals after a quick reference, and people trying to understand the evidence behind what they are reading elsewhere. If you value accuracy over hype and like seeing the source behind a claim, this was built for you.
What it is not
- Not a medical provider. Nothing here is medical advice, diagnosis, or treatment.
- We do not sell peptides. We do not sell, ship, or supply any research material.
- We do not endorse specific vendors. Supplier links are references for research use only products, not endorsements of any vendor's quality or practices.
Ownership and independence
DosingReference is independently run, and we want to be plain about the money. We do not sell peptides and hold no inventory. We earn affiliate commissions when readers buy through the supplier links on the site, including links to Quartz Peptides, and that is disclosed. No vendor pays us to write content, change a dose, or bury a safety note, and none has editorial approval over anything we publish. If our funding ever changes in a way that could affect our independence, we will update this.
Get in touch
Want to ask something, request a compound we have not covered, or flag an error? Reach out with the details, the compound name and vial size for a request, or the page and the issue for a correction, and we will take a look.